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Rabbit Anti-Human Bcl-2
Purified No Carrier Protein
Polyclonal Antibody
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Prod. No.:B445
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Bcl-2 recognizes an epitope in the intermediate domain of Human Bcl-2. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.2 Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis.2,3 These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis.4 It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad.5 Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.4,6

Known Species Cross Reactivity: Hu+
Immunogen
Bcl-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B471)
Blocking Peptide
Bcl-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B471)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Bcl-2 by Western blot analysis at a concentration of 1-2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A-20 Whole Cell Lysate (Leinco Prod. No.: A232).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.

3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.

4. Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
Related Products
Bcl-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B471)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A-20 Whole Cell Lysate (Leinco Prod. No.: A232)
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Anti-Human Bcl-2 (NT) (Leinco Prod. No.: B446)
Anti-Human Bcl-2 (Leinco Prod. No.: B589)
Anti-Human Bcl-2 (Leinco Prod. No.: B590)
Recombinant Human Bcl-2 (minus c-terminal) (Leinco Prod. No.: B548)
Anti-Human/Mouse Bcl-2 ( Antigen Aff. Pur. ) (Leinco Prod. No.: B642)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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