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Anti-Human CD5 (CD5)
Allophycocyanin (APC)
(Leu-1, Ly-1, T1, Tp67)
Monoclonal Antibody
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Prod. No.:C311
Clone:UCHT-2
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Description
Specificity:
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte antigen (Mr 67 kDa).

Antigen Distribution:
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 20 µl per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Flow Cytometry: This antibody can be used at a concentration of ≤ 20 µl per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1995) Oxford University Press
2. Leukocyte Typing III (1987) Oxford University Press
3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
Anti-Human CD5-Fluorescein (FITC) (Leinco Prod. No.: C115)
Anti-Human CD5-Biotin (Leinco Prod. No.: C114)
Anti-Mouse CD5-Fluorescein (FITC) (Leinco Prod. No.: C1529)
Anti-Mouse CD5-R-phycoerythrin (R-PE) (Leinco Prod. No.: C1530)
Anti-Human CD5 Purified (Leinco Prod. No.: C113)
Anti-Human CD5-R-phycoerythrin (R-PE) (Leinco Prod. No.: C116)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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