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Anti-Human CD5 (CD5)
Purified
(Leu-1, Ly-1, T1, Tp67)
Monoclonal Antibody
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Prod. No.:C113
Clone:UCHT-2
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).

Antigen Distribution:
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Background:
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD5. A suggested method would be to stain cells expressing CD5 with 1.0 µg per 106 cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1995) Oxford University Press
2. Leukocyte Typing III (1987) Oxford University Press
3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
Related Products
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Anti-Human CD5-Fluorescein (FITC) (Leinco Prod. No.: C115)
Anti-Human CD5-Biotin (Leinco Prod. No.: C114)
Anti-Mouse CD5-Fluorescein (FITC) (Leinco Prod. No.: C1529)
Anti-Mouse CD5-R-phycoerythrin (R-PE) (Leinco Prod. No.: C1530)
Anti-Human CD5-Allophycocyanin (APC) (Leinco Prod. No.: C311)
Anti-Human CD5-R-phycoerythrin (R-PE) (Leinco Prod. No.: C116)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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