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Rabbit Anti-Human/Mouse Bcl-2
Antigen Affinity Purified
Polyclonal Antibody
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Prod. No.:B642
Host:Rabbit
Pkg. Size:150 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Bcl-2 recognizes Human Bcl-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Purified Recombinant Human Bcl-2 (aa 4-22)(>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with ml of sterile 1X PBS pH 7.2-7.3 Containing 0.2% Sodium Azide (NaN3).
Reported Applications
WB
Western Blotting: To detect Human Bcl-2 this polyclonal antibody can be used at a concentration of 1.5 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Bcl-2 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human Bcl-2-related Protein A1 (Leinco Prod. No.: A409).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human Bcl-2-related Protein A1 (Leinco Prod. No.: A409)
Bcl-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B471)
Bcl-2 (NT) Blocking Peptide (Leinco Prod. No.: B472)
Anti-Human Bcl-2 (Intermediate Domain) (Leinco Prod. No.: B445)
Anti-Human Bcl-2 (NT) (Leinco Prod. No.: B446)
Anti-Human Bcl-2 (Leinco Prod. No.: B589)
Anti-Human Bcl-2 (Leinco Prod. No.: B590)
Recombinant Human Bcl-2 (minus c-terminal) (Leinco Prod. No.: B548)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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