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Anti-Mouse CD90 (CD90)
Allophycocyanin (APC)
(Thy-1)
Monoclonal Antibody
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Size


Prod. No.:C302
Clone:HK2.1
Isotype:Rat IgG2c
Conc.:0.1 mg/ml
Pkg. Size:100 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD90 (Clone HK2.1) recognizes the Thy-1.1 and Thy-1.2 alloantigens of all mouse strains tested. This antibody is reported to stimulate mouse T-cell proliferation.1

Antigen Distribution:
Anti-Mouse CD90 (Thy-1) is present on thymocytes, most peripheral T-lymphocytes, some intraepithelial T-lymphocytes, epithelial cells, fibroblasts, neurons and hematopoietic stem cells, but not B-cells of most mice tested.1,2

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Flow Cytometry: This antibody can be used at a concentration of ≤ 1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
2. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
3. Lancki, D. W. et al. (1984) Immunol. Rev. 81:65
Related Products
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Anti-Mouse CD90 (Thy 1, non-polymorphic)-Biotin (Leinco Prod. No.: C272)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-Fluorescein (Leinco Prod. No.: C273)
Anti-Mouse CD90 (Thy 1) (Activating) Purified (Leinco Prod. No.: C279)
Anti-Mouse CD90 (Thy 1) (Activating)-Fluorescein (Leinco Prod. No.: C281)
Anti-Mouse CD90 (Thy 1.2) Purified (Leinco Prod. No.: C283)
Anti-Mouse CD90 (Thy 1.2)-Biotin (Leinco Prod. No.: C284)
Anti-Mouse CD90 (Thy 1.2)-R-phycoerythrin (Leinco Prod. No.: C286)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-Allophycocyanin (Leinco Prod. No.: C356)
Anti-Mouse CD90 (Thy 1, non-polymorphic)-R-phycoerythrin (Leinco Prod. No.: C274)
Anti-Mouse CD90 (Thy 1, non-polymorphic) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C392)
Anti-Mouse CD90 (Thy 1.2) Low Endotoxin Functional Formulation (Leinco Prod. No.: C393)
Anti-Human CD90 (Thy-1)-Fluorescein (Leinco Prod. No.: C750)
Anti-Human CD90 (Thy-1)-R-phycoerythrin (Leinco Prod. No.: C751)
Anti-Mouse CD90 (Thy 1.2)-Allophycocyanin (Leinco Prod. No.: C357)
PN:
Anti-Mouse CD90 (Thy 1) (Activating) Low Endotoxin Functional Formulation (Leinco Prod. No.: C335)
Anti-Mouse CD90 (Thy 1) (Activating)-Biotin (Leinco Prod. No.: C280)
Anti-Mouse CD90 (Thy 1) (Activating)-R-phycoerythrin (Leinco Prod. No.: C282)
Anti-Mouse CD90 (Thy 1.2)-Fluorescein (Leinco Prod. No.: C285)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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