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Anti-Mouse Noggin (NOG)
Purified No Carrier Protein
Monoclonal Antibody
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Prod. No.:N216
Clone:426303
Isotype:Mouse IgG2b
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Mouse Noggin (Clone 426303) recognizes an epitope on Mouse Noggin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Known Species Cross Reactivity: Ms+
Host Species
Mouse
Immunogen
Purified Recombinant Mouse Noggin (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile to a final volume of 0.2 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Mouse Noggin is approximately 15 ng/well.
Western Blotting: To detect Mouse Noggin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse Noggin is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse Noggin (Leinco Prod. No.: N179).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse Noggin (Leinco Prod. No.: N179)
Anti-Mouse Noggin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N213)
Recombinant Human Noggin (Leinco Prod. No.: N142)
Anti-Mouse Noggin (Antigen Aff. Pur.) (Leinco Prod. No.: N207)
Recombinant Human Noggin (Leinco Prod. No.: N215)
Recombinant Mouse Noggin (Leinco Prod. No.: N182)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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