Product Datasheet
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Anti-Rat PCNA
DyLight® 800
Monoclonal Antibody
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Prod. No.:P337
Clone:PC10
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Rat PCNA (Clone PC10) recognizes an epitope on Rat PCNA. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.

Reporter Molecule:
DyLight® 800 conjugated antibodies absorb light maximally around 770 nm (± 4 nm) and fluoresce maximally around 794 nm. DyLight 800 is near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. This dye is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Rt+
Host Species
Mouse
Immunogen
Purified Recombinant Rat PCNA (>98%)
Formulation
This DyLight® 800 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 800 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121
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