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Goat Anti-Rat Leptin
Antigen Affinity Purified
(Obesity Protein (OB), B219, LEP, OBS)
Polyclonal Antibody
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Prod. No.:L154
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Rat Leptin recognizes Rat Leptin. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Leptin is a neuroendocrine hormone secreted from adipose tissue that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. Leptin is one of the most important adipose derived hormones.1 It interacts with six types of receptors and once it binds to OB-Receptor it activates the stat3, which is phosphorylated and travels to the nucleus to effect changes in gene expression. One of the main effects on gene expression is the down-regulation of the expression of endocannabinoids.2 High leptin levels are associated with psoriasis.3

Immunogen
Purified Recombinant Rat Leptin (>98%)
Formulation
This antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this antigen affinity purified polyclonal antibody using sterile deionized water to a concentration >0.2 mg/ml.
Storage and Stability
The lyophilized antibody should be stored desiccated at -20°C. The reconstituted antibody can be stored for at least six weeks at 4°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Rat Leptin is approximately 0.2 ng/well.
Neutralization: Not currently tested.
Western Blotting: To detect Rat Leptin this polyclonal antibody can be used at a concentration of 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Rat Leptin is 1.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Mantzoros CS et al.(2006) Nat Clin Pract Endocrinol Metab. 2: 318
2. Hill RA. et al.(2002) Int. J. Obes. Relat. Metab. Disord. 26: 1407
3. Chen Y-J. et al.(2008) Arch Derm. 144: 1571
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Canine Leptin (Leinco Prod. No.: L182)
Recombinant Rabbit Leptin (Leinco Prod. No.: L183)
Anti-Human Leptin (Antigen Aff. Pur.) (Leinco Prod. No.: L122)
Anti-Mouse Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L111)
Recombinant Mouse Leptin (Leinco Prod. No.: L117)
Anti-Human Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L110)
Anti-Rat Leptin-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L155)
Recombinant Porcine Leptin (Leinco Prod. No.: L180)
Anti-Mouse Leptin (Antigen Aff. Pur.) (Leinco Prod. No.: L115)
Recombinant Bovine Leptin (Leinco Prod. No.: L178)
Recombinant Human Leptin (Leinco Prod. No.: L116)
Recombinant Equine Leptin (Leinco Prod. No.: L179)
Recombinant Chicken Leptin (Leinco Prod. No.: L181)
Human Leptin ELISA Development Kit (Leinco Prod. No.: L234)
Anti-Human Leptin (Leinco Prod. No.: L109)
Mouse Leptin ELISA Development Kit (Leinco Prod. No.: L236)
Recombinant Rat Leptin (Leinco Prod. No.: L146)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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