UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.
DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Chicken Egg White
UltraAvidin™-DyLight® 650 is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Chicken Egg White
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
11-17 µg biotin bound/mg UltraAvidin™
pI= 6.3 +/- 0.3
250,000 ε(M-1 cm-1)
Applications and Suggested Working Dilutions
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000
However, each investigator should determine their own optimal working dilution for each specific research application.
Country of Origin
1. Alon, R., Bayer, E. A., and Wilchek, M.1 (1990) "Streptavidin Contains An RYD Sequence Which Mimics The RGD Receptor Domain Of Fibronectin" Biochemical and Biophysical Research Communications 170:1236-1241
2. A. F. Dernburg, D. R. Daily, K. J. Yook, J. A. Corbin, J. W. Sedat, and W. Sullivan, (1996) “Selective Loss of Sperm Bearing a Compound Chromosome in the Drosophila Female" Genetics 143: 1629 - 1642
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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