Background:

UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.