Background:

UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

DyLight® 405 conjugated antibodies are excited maximally at about 400 nm (± 4 nm) and fluoresce with a peak at about 420 nm. DyLight® 405 is not recommended for use in epifluorescence microscopes, nor is it recommended for flow cytometry, because emission filters commonly used in flow cytometers are not optimal for DyLight® 405. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.