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UltraAvidin™- PerCP
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Prod. No.:A430
Conc.:1.0 mg/ml
Pkg. Size:1.0 mg, 2.0 mg
Storage:2-8°C Detailed storage instructions below.
Description
Background:
UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.


Reporter Molecule:
PerCp is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. PerCP is a protein complex with a molecular weight of ~35 kDa. Due to its photobleaching characteristics do not use on flow cytometers with high-power lasers.

Host Species
Chicken Egg White
Formulation
UltraAvidin™-PerCP is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Source
Chicken Egg White
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
Specific Activity
11-17 µg biotin bound/mg UltraAvidin™
Isoelectric Point
pI= 6.3 +/- 0.3
Extinction Coeffecient
40,600 ε(M-1 cm-1)
Applications and Suggested Working Dilutions
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.
Country of Origin
USA
References
1. Alon, R., Bayer, E. A., and Wilchek, M.1 (1990) "Streptavidin Contains An RYD Sequence Which Mimics The RGD Receptor Domain Of Fibronectin" Biochemical and Biophysical Research Communications 170:1236-1241

2. A. F. Dernburg, D. R. Daily, K. J. Yook, J. A. Corbin, J. W. Sedat, and W. Sullivan, (1996) “Selective Loss of Sperm Bearing a Compound Chromosome in the Drosophila Female" Genetics 143: 1629 - 1642
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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