Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
AMCA is a small fluorescent organic molecule with an absorbance maximum of 350 nm and an emission maximum of 450 nm.
Streptavidin-AMCA is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, and 0.09% sodium azide as a preservative.
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
Applications and Suggested Working Dilutions
The suggested working concentration for most applications for Streptavidin-AMCA is 2-5 μg/ml.
Country of Origin
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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