Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
Reporter Molecule:
PerCp is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. PerCP is a protein complex with a molecular weight of ~35 kDa. Due to its photobleaching characteristics do not use on flow cytometers with high-power lasers.
Host Species
Streptomyces avidinii
Formulation
Streptavidin-PerCP is supplied in 0.01M phosphate buffered saline (PBS), 0.25M NaCl, pH 7.6, containing 15 mg/ml BSA and 0.05% sodium azide as a preservative.
Source
Streptomyces avidinii
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.