5:Streptavidin-AMCA


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Streptavidin-AMCA
SizeQuantityTotal
1.0 mg
Prod. No.:S212
Conc.:1.0 mg/ml
Pkg. Size:1.0 mg
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Reporter Molecule:
AMCA is a small fluorescent organic molecule with an absorbance maximum of 350 nm and an emission maximum of 450 nm.

Host Species
Streptomyces avidinii
Formulation
Streptavidin-AMCA is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, and 0.09% sodium azide as a preservative.
Source
Streptomyces avidinii
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
Applications and Suggested Working Dilutions
The suggested working concentration for most applications for Streptavidin-AMCA is 2-5 μg/ml.
Country of Origin
USA
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