Streptavidin-Alkaline Phosphatase (Alk Phos)
| Prod. No.: | S208 |
| Conc.: | 1.0 mg/ml |
| Pkg. Size: | 1.0 mg, 2.0 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
Reporter Molecule:
Alkaline Phosphatase (Alk Phos) is an enzyme purified from bovine calf intestinal mucosa with a molecular weight of approximately 140 kDa.
Host Species
Streptomyces avidinii
Formulation
Streptavidin-Alk. Phos. is supplied in 0.01M Tris pH 8.0, containing .25 NaCl, 30 mg/ml BSA and 0.05% sodium azide as a preservative.
Source
Streptomyces avidinii
Storage and Stability
This Alkaline Phosphatase conjugate is stable for ~6 weeks when stored at 2-8°C. Do not freeze. For extended storage, add an equal volume of ACS grade or better glycerol for a final concentration of 50%, then store at -20°C.
Applications and Suggested Working Dilutions
Western blotting: 0.1 - 1.0 µg/ml
ELISA: 1 - 2 µg/ml
Immunohistochemistry: 1 - 2 µg/ml
Western blotting and ELISA Chemiluminescent: 0.1 - 0.05 µg/ml
However, each investigator should determine their own optimal working dilution for each specific research application.
Country of Origin
USA
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
