Streptavidin-Cy5
| Prod. No.: | S214 |
| Conc.: | 1.8 mg/ml |
| Pkg. Size: | 1.0 mg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
Reporter Molecule:
Cyanine 5 is a small fluorescent organic molecule with an absorbance maximum of 650 nm and an emission maximum of 670 nm.
Formulation
Streptavidin-Fluorescein is supplied in 0.01M phosphate buffered saline (PBS), 0.25M NaCl, pH 7.6, containing 15 mg/ml BSA and 0.05% sodium azide as a preservative.
Reconstitution
It is recommended to reconstitute the Streptavidin-Cy5 with 0.65 ml deionized water, for a final concentration of 1.8 mg/ml.
Storage and Stability
The lyophilized streptavidin is stable when stored at 2-8°C. After reconstitution the streptavidin may be stored as an undiluted liquid at 2-8°C for six weeks. For extended storage as a liquid an equal volume of ACS grade or better glycerol should be added to the vial (bringing the final concentration of streptavidin to 50%) and stored at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. The reconstituted material may also be aliquoted and frozed at -70°C without the addition of glycerol.
Applications and Suggested Working Dilutions
0.5 - 2.0 µg/ml
Country of Origin
USA
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
