Streptavidin-Fluorescein (FITC)
| Prod. No.: | S205 |
| Conc.: | 1.0 mg/ml |
| Pkg. Size: | 1.0 mg, 5.0 mg |
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.
Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.
Host Species
Streptomyces avidinii
Formulation
Streptavidin-Fluorescein is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Source
Streptomyces avidinii
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Specific Activity
≥ 14.0 U/mg
Isoelectric Point
pI = 5 - 6
Applications and Suggested Working Dilutions
Streptavidin-FITC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A woring dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications.
Country of Origin
USA
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
