5:Streptavidin-Texas Red


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Streptavidin-Texas Red
SizeQuantityTotal
Prod. No.:S209
Conc.:1.0 mg/ml
Pkg. Size:1.0 mg, 5.0 mg
Storage:2 - 8°C Detailed storage instructions below.
Description
Background:
Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Reporter Molecule:
Texas Red is a small long wavelength fluorescent molecule with a molecular weight of 625 daltons with an absorbance maximum of ~589 nm and an emission maximum of ~615 nm.

Host Species
Streptomyces avidinii
Formulation
Streptavidin-Texas Red is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, 1.0% Glycerol and 0.09% sodium azide as a preservative.
Source
Streptomyces avidinii
Storage and Stability
Streptavidin-Texas Red is stable when stored at 2 - 8°C. Do not freeze
Specific Activity
≥ 14.0 U/mg
Isoelectric Point
pI = 5 - 6
Applications and Suggested Working Dilutions
Flow Cytometry: ≤ 1.0 µg/1.0 X 106 cells in a volume of 100 µl.
Microscopy: 0.1 - 2.0 µg/ml
Country of Origin
USA
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