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Streptavidin-Texas Red | Prod. No.: | S209 | | Conc.: | 1.0 mg/ml | | Pkg. Size: | 1.0 mg, 5.0 mg | | Storage: | 2 - 8°C Detailed storage instructions below. |
Description Background: Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10
-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin. Reporter Molecule: Texas Red is a small long wavelength fluorescent molecule with a molecular weight of 625 daltons with an absorbance maximum of ~589 nm and an emission maximum of ~615 nm. Host Species Streptomyces avidinii Formulation Streptavidin-Texas Red is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, 1.0% Glycerol and 0.09% sodium azide as a preservative.
Source Streptomyces avidinii Storage and Stability Streptavidin-Texas Red is stable when stored at 2 - 8°C. Do not freeze
Specific Activity ≥ 14.0 U/mg Isoelectric Point pI = 5 - 6 Applications and Suggested Working Dilutions Flow Cytometry: ≤ 1.0 µg/1.0 X 106 cells in a volume of 100 µl.
Microscopy: 0.1 - 2.0 µg/ml
Country of Origin USA Related Products
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