UltraAvidin™-Texas Red
| Prod. No.: | A109 |
| Conc.: | 1.0 mg/ml |
| Pkg. Size: | 1.0 mg, 5.0 mg |
Description
Background:
UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000, there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a neutral pl which prevents electrostatic interactions with negatively charged serum or membrane proteins..
Recent studies have identified a universal recognition sequence in streptavidin which is similar to that found in several adhesion receptors.3 Strong interactions have been shown with streptavidin and cell surface molecules. This universal recognition sequence is not present in UltraAvidin. UltraAvidin may not significantly increase the sensitivity in all research systems but it will assure specificity.
Reporter Molecule:
Texas Red is a small long wavelength fluorescent molecule with a molecular weight of 625 daltons with an absorbance maximum of ~589 nm and an emission maximum of ~615 nm.
Host Species
Chicken Egg White
Formulation
UltraAvidin-Texas Red is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, 1.0% Glycerol and 0.09% sodium azide as a preservative.
Source
Chicken Egg White
Storage and Stability
UltraAvidin-Texas Red is stable when stored at 2 - 8°C. Do not freeze
Specific Activity
11-17 µg biotin bound/mg UltraAvidin™
Isoelectric Point
pI= 6.3 +/- 0.3
Applications and Suggested Working Dilutions
UltraAvidin-Texas Red can be used to detect biotinylated biological probes such as antibodies or cytokines in a variety of applications such as flow cytometry or fluorescent microscopy..
Leinco Technologies suggests a working dilution of approximately 1:500
for immunohistology and ≤ 1.0 microgram to stain 1.0 X 106 cells in 100 µl in flow cytometric applications. A woring dilution of ≤ 1.0 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications. However, each investigator should determine their own optimal working dilution for each specific research application.
Country of Origin
USA
References
1. Alon, R., Bayer, E. A., and Wilchek, M.1 (1990)"Streptavidin Contains An RYD Sequence Which Mimics The RGD Receptor Domain Of Fibronectin" Biochemical and Biophysical Research Communications 170:1236-1241
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
