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Bcl-B Blocking Peptide
(Boo, Diva, Nrh )
Blocking Peptide
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Size


Prod. No.:B473
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Bcl-B peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
16 amino acids near the amino terminus of human Bcl-B.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Bcl-B (Leinco Prod. No.: B447) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53.

2. Kouzarides T. Histone methylation in transcriptional control. Curr. Opin. Genet. Dev. 2002; 12:198-209.

3. Shi YJ, Matson C, Lan F, et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol. Cell 2005; 19:857-64.

4. Nakamura T, Mori T, Tada S, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol. Cell 2002; 10:1119-28.
Related Products
Anti-Human Bcl-B (Leinco Prod. No.: B447)
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