Bcl-B Blocking Peptide [B473] : Leinco Technologies, excellence in early discovery research

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Bcl-B Blocking Peptide

(Boo, Diva, Nrh )

Blocking Peptide

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Size

50 µg ( $115.00 )

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Prod. No.:	B473
Conc.:	0.2 mg/ml
Pkg. Size:	50 µg
Storage:	-20°C Detailed storage instructions below.

Description

Background:

Bcl-B peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location

16 amino acids near the amino terminus of human Bcl-B.

Formulation

This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage and Stability

Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

IHC (NBF/Par.), N, WB

Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Bcl-B (Leinco Prod. No.: B447) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Shi Y, Lan F, Matson C, et al. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 2004; 119:941-53.

2. Kouzarides T. Histone methylation in transcriptional control. Curr. Opin. Genet. Dev. 2002; 12:198-209.

3. Shi YJ, Matson C, Lan F, et al. Regulation of LSD1 histone demethylase activity by its associated factors. Mol. Cell 2005; 19:857-64.

4. Nakamura T, Mori T, Tada S, et al. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation. Mol. Cell 2002; 10:1119-28.