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Caspase-12 (Intermediate Domain) Blocking Peptide
Blocking Peptide
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Size


Prod. No.:C1132
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Caspase-12 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
This peptide sequence is located at amino acids 100 to 116 of Mouse Caspase-12 protein.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Caspase-12 (Intermediate Domain) (Leinco Prod. No.: C1115) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998;21:230-5

2.Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G. BNIP3α: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res 1999;59:533-7

3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10

4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999;18:4523-9 (RD1200)

5. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmicreticulum-specific apoptosis and cytotoxicity by amyloid -beta. Nature 2000;403:98-103.
Related Products
Anti-Human Caspase-12 (Intermediate Domain) (Leinco Prod. No.: C1115)
Caspase-12 (NT) Blocking Peptide (Leinco Prod. No.: C1128)
Anti-Human Caspase-12 (large cleavage product) (Leinco Prod. No.: C1247)
Anti-Human Caspase-12 (small cleavage product) (Leinco Prod. No.: C1248)
Caspase-12 (large) Blocking Peptide (Leinco Prod. No.: C1283)
Caspase-12 (small) Blocking Peptide (Leinco Prod. No.: C1285)
Anti-Human Caspase-12 (NT) (Leinco Prod. No.: C1116)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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