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Caspase-12 (NT) Blocking Peptide
Blocking Peptide
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Size


Prod. No.:C1128
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:-20°C Detailed storage instructions below.
Description
Background:
Caspase-12 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location
This peptide sequence is located at amino acids 2 to 17 of Mouse Caspase-12 protein.
Formulation
This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Storage and Stability
Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), N, WB
Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human Caspase-12 (NT) (Leinco Prod. No.: C1116) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8.

2.Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J. Murine Ortholog of ASC, a CARD-Containing Protein, Self-Associates and Exhibits Restricted Distribution in Developing Mouse Embryos. Exp Cell Res. 2001;262(2):128-133.

3. Conway KE, McConnell BB, Bowring CE, Donald CD, Warren ST , Vertino PM. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 2000;60(22):6236-42.

4. McConnell BB, Vertino PM. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res. 2000;60(22):6243-7. (WD0102)

5. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmicreticulum-specific apoptosis and cytotoxicity by amyloid -beta. Nature 2000;403:98-103.
Related Products
Anti-Human Caspase-12 (NT) (Leinco Prod. No.: C1116)
Caspase-12 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1132)
Anti-Human Caspase-12 (large cleavage product) (Leinco Prod. No.: C1247)
Anti-Human Caspase-12 (small cleavage product) (Leinco Prod. No.: C1248)
Caspase-12 (large) Blocking Peptide (Leinco Prod. No.: C1283)
Caspase-12 (small) Blocking Peptide (Leinco Prod. No.: C1285)
Anti-Human Caspase-12 (Intermediate Domain) (Leinco Prod. No.: C1115)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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