DDX3 (Intermediate Domain) Blocking Peptide [D327] : Leinco Technologies, excellence in early discovery research

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DDX3 (Intermediate Domain) Blocking Peptide

(Dead Box Polypeptide 3 X-Linked, DBX, Helicase-Like Protein 2 )

Blocking Peptide

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Size

50 µg ( $115.00 )

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Prod. No.:	D327
Conc.:	0.2 mg/ml
Pkg. Size:	50 µg
Storage:	-20°C Detailed storage instructions below.

Description

Background:

DDX3 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

Amino Acid Location

16 amino acids near the center of human DDX3.

Formulation

This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.

Storage and Stability

Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

Indirect ELISA, N

Neutralization: The peptide may be used to neutralize the binding activity of Anti-Human DDX3 (Intermediate Domain) (Leinco Prod. No.: D325) by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2.

2. Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.

3. Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

4. Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.