Ramos Whole Cell Lysate
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DetailsCell Line Ramos (Burkitt's Lymphoma) Growth Media ATCC Medium (RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, and 10% heat-inactivated FBS.) Preparation Ramos lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. Host Human Concentration 2.0 mg/ml Formulation The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol. Expiration Date One year from date of receipt Storage This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C <b>in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.</b> For longer term storage, freeze working aliquots at -70°C. Applications and Suggested Working Dilutions This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.