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Anti-Human CD10 (CD10)
Allophycocyanin (APC)
(CALLA)
Monoclonal Antibody
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Prod. No.:C305
Clone:SJ5-1B4
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD10 (Clone SJ5-1B4) recognizes Human CD10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD10 (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen. CD10 is a (Mr 100 kDa) surface glycoprotein with associated neutral endopeptidase activity.1,2

Antigen Distribution:
The CD10 antigen is found on cells from subjects with acute B-lymphoid leukemia.3 The antigen is also expressed in various degrees on progenitor B-cells, renal epithelium, fibroblasts, granulocytes, early T-lymphocytes and germinal center cells.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hux
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing V (1995) Oxford University Press
2. Billings, R. et al. (1978) J. Natl. Cancer Inst. 61:583
3. Ritz, J. et al. (1980) Nature 283:583
4. Foon, K. A. et al. (1982) Blood 60:1
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
PN:
Anti-Human CD10 (CALLA) Purified (Leinco Prod. No.: C137)
Anti-Human CD10 (CALLA)-Biotin (Leinco Prod. No.: C138)
Anti-Human CD10 (CALLA)-Fluorescein (FITC) (Leinco Prod. No.: C139)
Anti-Human CD10 (CALLA)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C140)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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