Anti-Human CD16
Purified
(FcγRIII)
Monoclonal Antibody
| Prod. No.: | C465 |
| Clone: | GRM1 |
| Isotype: | Mouse IgG2a |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 0.1 mg |
Description
Specificity:
Mouse Anti-Human CD16 (Clone GRM1) recognizes Human CD16. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The CD16 molecule has been described as the low affinity Fc receptor (FcRIII) for complexed IgG which may exist either as a transmembranous form or as glycosyl-phosphatidylinositol form. It is expressed on NK cells, granulocytes (PMN) and macrophages. The GPI-linked form is also expressed on neutrophils.
Antigen Distribution:
Granulocytes: >98%
LGL cells: >85%
Peripheral blood lymphocytes: 12 + 8%
Thymocytes: <1%
Monocytes: <1%
B cells (CD20+): <1%
T cells (CD3+): <1%
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This purified monoclonal antibody is formulated in phosphate buffered saline (PBS), 1 mg/ml BSA and 10 mM sodium azide as a preservative.
Storage and Stability
For use within 1 month of purchase store at 2-8°C. For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FC, IP, WB
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Leukocyte Typing V (1995) Oxford University Press
2. Leukocyte Typing IV (1989) Oxford University Press
3. Leukocyte Typing III (1987) Oxford University Press
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
