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Anti-Human CD19 (CD19)
Allophycocyanin (APC)
(Pan B-Cells)
Monoclonal Antibody
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Prod. No.:C317
Clone:SJ25-C1
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Description
Specificity:
Mouse Anti- CD19 (Clone SJ25-C1) recognizes CD19. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD19 recognizes a (Mr 90 kDa) B-cell surface glycoprotein which is present at all stages of maturation. Anti-CD19 does not react with T-cells, granulocytes, monocytes, erythrocytes or platelets.

Antigen Distribution:
The CD19 antigen is expressed on approximately 5-25% of human peripheral blood lymphocytes and approximately 60% of splenic lymphocytes.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing III (1987) Oxford University Press: Code No.: 073
2. Leukocyte Typing II (1986) Oxford University Press
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
Anti-Human CD19 (Pan B-Cells)-Biotin (Leinco Prod. No.: C573)
Anti-Human CD19 (Pan B-Cells)-Fluorescein (Leinco Prod. No.: C574)
Anti-Human CD19 (Pan B-Cells)-R-phycoerythrin (Leinco Prod. No.: C575)
Anti-Human CD19 (Pan B-Cells)-R-phycoerythrin/Cy5 (Leinco Prod. No.: C195)
Anti-Human CD19 (Pan B-Cells)-R-phycoerythrin/Cy5 (Leinco Prod. No.: C576)
Anti-Human CD19 (Pan B-Cells) Purified (Leinco Prod. No.: C141)
Anti-Human CD19 (Pan B-Cells)-Biotin (Leinco Prod. No.: C142)
Anti-Human CD19 (Pan B-Cells) Purified (Leinco Prod. No.: C572)
Anti-Human CD19 (Pan B-Cells)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C144)
PN:
Anti-Human CD19 (Pan B-Cells)-Fluorescein (FITC) (Leinco Prod. No.: C143)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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