Anti-Human CD2
Allophycocyanin (APC)
Monoclonal Antibody
| Prod. No.: | C308 |
| Clone: | G11 |
| Isotype: | Mouse IgG2a |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.
Antigen Distribution:
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.
Host Species
Mouse
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
FC: This antibody can be used at a concentration of ≤1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Meuer, S.C. et al. (1984) Cell 36:897
2. Peterson, A. and Sneed, B. (1987) Nature 329:842
3. Bierer, B.E. et al. (1989) Annu. Rev Immunol. 7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489
5. Krensky, C.F. et al. (1983) J. Immunol. 131:611
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
