Anti-Human CD2
Biotin
Monoclonal Antibody
| Prod. No.: | C110 |
| Clone: | G11 |
| Isotype: | Mouse IgG2a |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg , 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.
Antigen Distribution:
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Background:
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD2. A suggested method would be to stain cells expressing CD2 with Anti-Human CD2 at ≤1 µg per 1.0 X 10^6 cells in a 100 µl total staining volume, followed by UltraAvidin™- R-phycoerythrin (R-PE) (
Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Meuer, S.C. et al. (1984) Cell 36:897
2. Peterson, A. and Sneed, B. (1987) Nature 329:842
3. Bierer, B.E. et al. (1989) Annu. Rev Immunol. 7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489
5. Krensky, C.F. et al. (1983) J. Immunol. 131:611
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
