Anti-Human CD2
Purified Low Endotoxin Functional Formulation
Monoclonal Antibody
| Prod. No.: | C365 |
| Clone: | G11 |
| Isotype: | Mouse IgG2a |
| Conc.: | 1.0 - 5.0 mg/ml |
| Pkg. Size: | Custom, 100 µg, 500 µg, 1 mg |
| Storage: | Sterile 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.
Antigen Distribution:
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Background:
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
Host Species
Mouse
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Meuer, S.C. et al. (1984) Cell 36:897
2. Peterson, A. and Sneed, B. (1987) Nature 329:842
3. Bierer, B.E. et al. (1989) Annu. Rev Immunol. 7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489
5. Krensky, C.F. et al. (1983) J. Immunol. 131:611
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
