Anti-Human CD2
R-phycoerythrin (R-PE)
Monoclonal Antibody
| Prod. No.: | C112 |
| Clone: | G11 |
| Isotype: | Mouse IgG2a |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD2 (Clone G11) recognizes Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD2 recognizes a cell surface glycoprotein (Mr 45-59 kDa) on human lymphocytes. This antigen also forms the binding site for sheep erythrocytes.
Antigen Distribution:
The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.
Background:
Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.1 The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),2 the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).3 Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.
Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.
Host Species
Mouse
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
FC: This antibody can be used at a concentration of ≤1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Meuer, S.C. et al. (1984) Cell 36:897
2. Peterson, A. and Sneed, B. (1987) Nature 329:842
3. Bierer, B.E. et al. (1989) Annu. Rev Immunol. 7:579
4. Krensky, C.F. et al. (1982) Proc. Natl. Acad. Sci. USA 79:7489
5. Krensky, C.F. et al. (1983) J. Immunol. 131:611
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
