Anti-Human CD3
Biotin
Monoclonal Antibody
| Prod. No.: | C106 |
| Clone: | UCHT-1 |
| Isotype: | Mouse IgG1 |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a human T-lymphocyte surface glycoprotein (Mr 22-28 kDa).1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR).2
Antigen Distribution:
The CD3 antigen is present on 60-80% of normal peripheral blood lymphocytes, 20-40% of normal spleen cells, the majority of T-CLL and approximately 70% of T‑ALL cells.
Background:
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This pan T-cell marker has also been used to determine T- versus B-cell lymphomas and leukemias.
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with Anti-Human CD3 at ≤1.0 ug per 1.0 X 10
6 cells in a 100 ul total staining volume, followed by, followed by UltraAvidin™- R-phycoerythrin (R-PE) (
Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Meuer, S. C. et al. (1983) Nature 303:808
4. Beverly, P.C.L. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
