Anti-Human CD3
Fluorescein (FITC)
Monoclonal Antibody
| Prod. No.: | C107 |
| Clone: | UCHT-1 |
| Isotype: | Mouse IgG1 |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein.1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR).2
Antigen Distribution:
The human CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL cells.
Background:
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.
Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.
Host Species
Mouse
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
FC: This antibody can be used at a concentration of ≤1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Meuer, S. C. et al. (1983) Nature 303:808
4. Beverly, P.C.L. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
