Anti-Human CD3
Purified Low Endotoxin Functional Formulation
Monoclonal Antibody
| Prod. No.: | C364 |
| Clone: | UCHT-1 |
| Isotype: | Mouse IgG1 |
| Conc.: | 1.0 mg/ml |
| Pkg. Size: | Custom, 100 µg, 500 µg |
| Storage: | Sterile 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein.1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR).2
Antigen Distribution:
The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
Background:
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.
Host Species
Mouse
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Meuer, S. C. et al. (1983) Nature 303:808
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
