Anti-Human CD5
Biotin
Monoclonal Antibody
| Prod. No.: | C114 |
| Clone: | UCHT-2 |
| Isotype: | Mouse IgG1 |
| Conc.: | 0.1 mg/ml |
| Pkg. Size: | 50 µg, 100 µg, 200 µg |
| Storage: | 2-8°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte antigen (Mr 67 kDa).
Antigen Distribution:
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Background:
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
Host Species
Mouse
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD5. A suggested method would be to stain cells expressing CD5 with Anti-Human CD5 at ≤1 µg per 1.0 X 10
6 cells in a 100 µl total staining volume, followed by UltraAvidin™- R-phycoerythrin (R-PE) (
Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Leukocyte Typing VI (1995) Oxford University Press
2. Leukocyte Typing III (1987) Oxford University Press
3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids," Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
