Specificity:

Mouse Anti-Human CD57 (Clone VC1.1) recognizes Human CD57. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-CD57 recognizes a (Mr 110 kD) carbohydrate molecule on myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG).¹ This antibody blocks binding of HNK-1 (Leu. 7) antibody.³

The CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.

Background:

Anti-CD57 may be used in flow cytometry, Western blotting and immunohistology on formalin fixed paraffin embedded tissue sections. The antibody is useful for enumeration of NK subsets and T-lymphocyte subsets, studies of neural tissue, malignant tissues and AIDS-related complex.

R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.

Known Species Cross Reactivity: Hu+