Mouse Anti-Human CD8 (CD8) (Clone UCHT-4) recognizes an epitope on Human CD8. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD8 recognizes a T-cell glycoprotein cell surface complex (Mr 32 kDa)1 which defines the cytotoxic/suppressor population of mature T-lymphocytes.2 The CD8 complex interacts with class I MHC molecules resulting in increased adhesion between CD8+ T-lymphocytes and their target cells. Binding of the CD8 antigen to Class I MHC molecules enhances T-cell activation.3 The cytoplasmic portion of the CD8 antigen is associated with a protein tyrosine kinase (p56lck).4
The CD8 antigen is expressed on approximately 19-48% of normal human peripheral blood lymphocytes and >90% of normal thymocytes. It is also present on a subset of NK cells. Subjects infected with HIV have been shown to exhibit increased levels of CD8+ Lymphocytes with a continual decrease in CD4+ lymphocytes.
Anti-CD8-Biotin may be used in studies of immunoregulation and T-lymphocyte mediated suppression, autoimmune disorders, immunodeficiency states and acute lymphoblastic leukemia and lymphoma.
Known Species Cross Reactivity: Hu+
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD8. A suggested method would be to stain cells expressing CD8 with 0.03 µg per million cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
1. Parnes, J. R. et al. (1989) Adv. Immunol.44:265
2. Reinherz, E. L. et al. (1980) J. Immunol.124:1301
3. Fischer, A. et al. (1983) Immunology48:177
4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol.18:1653
5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31