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Anti-Human Ephrin-A3

Purified No Carrier Protein

(Ehk1-L, EFL-2, LERK-3, EFNA3, EPLG3 )

Monoclonal Antibody

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Prod. No.:	E247
Clone:	80814.11
Isotype:	Mouse IgG_2a
Pkg. Size:	500 µg, 1 mg
Storage:	-20°C to -70°C Detailed storage instructions below.

Description

Specificity:

Mouse Anti-Human Ephrin-A3 (Clone 80814.11) recognizes an epitope on Human Ephrin-A3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 5% cross-reactivity with rhEphrin-A5 and no cross-reactivity with rhEphrin-A4, rmEphrin-A1, rmEphrin-B1, rhEphrin-B2 and rhEphrin-B3.

Background:

Ephrin-A3, also known as EFNA3 encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin-A3 encodes an EFNA class ephrin.

Known Species Cross Reactivity: Hu+

Host Species

Mouse

Immunogen

Purified Recombinant Human Ephrin-A3 (>98%)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Reconstitution

This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.

Endotoxin

<0.1 EU/µg as determined by the LAL method

Storage and Stability

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Reported Applications

Indirect ELISA, WB

Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human Ephrin-A3 is approximately 1.2 ng/well.

Western Blotting: To detect Human Ephrin-A3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Ephrin-A3 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human Ephrin-A3 (Leinco Prod. No.: E198).

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA