Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Mouse CD24 (CD24)
Allophycocyanin (APC)
(Heat Stable Antigen, Nectadrin)
Monoclonal Antibody
Share |


Prod. No.:C343
Clone:R7129.7
Conc.:0.1 mg/ml
Pkg. Size:25 µg, 100 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD24 (Clone R7129.7) recognizes the cell surface glycoprotein Nectadrin, immunologically identical to the heat stable antigen (HSA). Nectadrin is a glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of Nectadrin purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kD.

Antigen Distribution:
The CD24 (Nectadrin) antigen is present on lymphocytes, granulocytes, erythrocytes.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 1 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lesley, J. et al. (1988) Cell Immunol. 112:40
2. Kadmon, G. et al. (1992) J. Cell Biol. 118(5):1245
3. Alterman, L. A. et al. (1990) Eur. J. Immunol. 20(7):1597
4. Wegner, R. H. et al. (1993) J. Biol. Chem. 268:23345
Related Products
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-R-phycoerythrin (Leinco Prod. No.: C238)
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-Biotin (Leinco Prod. No.: C236)
PN:
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin) Purified (Leinco Prod. No.: C235)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2013