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Anti-Mouse CD24 (CD24)
Purified
(Heat Stable Antigen, Nectadrin)
Monoclonal Antibody
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Prod. No.:C235
Clone:R7129.7
Isotype:Rat IgG
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 500 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD24 (Clone R7129.7) recognizes the cell surface glycoprotein Nectadrin, immunologically identical to the heat stable antigen (HSA). Nectadrin is a glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of Nectadrin purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kD.

Antigen Distribution:
The CD24 (Nectadrin) antigen is present on lymphocytes, granulocytes, erythrocytes.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD24. A suggested method would be to stain cells expressing CD24 with 1 µg per 1 x 106 cells in a total staining volume of 100 µl followed by Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lesley, J. et al. (1988) Cell Immunol. 112:40
2. Kadmon, G. et al. (1992) J. Cell Biol. 118(5):1245
3. Alterman, L. A. et al. (1990) Eur. J. Immunol. 20(7):1597
4. Wegner, R. H. et al. (1993) J. Biol. Chem. 268:23345
Related Products
Goat F(ab')2 Anti-Rat IgG, Fc (Gamma) Fragment Specific-R-phycoerythrin (Leinco Prod. No.: R485)
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-R-phycoerythrin (Leinco Prod. No.: C238)
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-Biotin (Leinco Prod. No.: C236)
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-Allophycocyanin (Leinco Prod. No.: C343)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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