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Anti-Mouse CD3ε (CD3ε)
Purified Low Endotoxin Functional Formulation
(CD3e)
Monoclonal Antibody
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Prod. No.:C219
Clone:500A2
Isotype:Syrian Hamster IgG2a κ
Conc.:1.0 mg/ml
Pkg. Size:50 µg, 500 µg, 1 mg
Storage:Sterile 2-8°C Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse CD3ε (Clone 500 A2) recognizes an epitope on Mouse CD3ε. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Mouse T-cell receptor
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FA
Functional Assay: Binding of Hamster Anti-Mouse CD3ε (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse CD3ε-Biotin (Leinco Prod. No.: C208)
Anti-Mouse CD3ε Purified (Leinco Prod. No.: C207)
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