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Anti-Mouse CD8a (CD8a)
Biotin
(Ly2)
Monoclonal Antibody
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Prod. No.:C216
Clone:53-6.7
Isotype:Rat IgG2a
Conc.:0.5 mg/ml
Pkg. Size:100 µg, 500 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD8a (Clone 53-6.7) recognizes Lyt 2, lymphocyte differentiation antigen. The antibody precipitates 44 kDa and 33 kDa proteins and reacts with non-polymorphic determinant.

Antigen Distribution:
Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.1 CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
IHC (Frozen), FC
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD8a. A suggested method would be to stain cells expressing CD8a with 0.25 µg per 1 x 106 cells in a total staining volume of 100 µl followed by UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Nakayama, K. et al. (1994) Science 263:1131
2. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
3. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
4. Van Ewijk, et al. (1981) J. Immunol. 127:2594
Related Products
UltraAvidin™- R-phycoerythrin (R-PE) (Leinco Prod. No.: A104)
Anti-Mouse CD8a (Ly 2) (All Alleles)-Fluorescein (Leinco Prod. No.: C217)
Anti-Mouse CD8a (Ly 2.2)-R-phycoerythrin (Leinco Prod. No.: C300)
Anti-Mouse CD8a (Ly 2) (All Alleles)-R-phycoerythrin (Leinco Prod. No.: C218)
Anti-Mouse CD8a (Ly 2.2) Purified (Leinco Prod. No.: C297)
Anti-Mouse CD8a (Ly 2.2)-Fluorescein (Leinco Prod. No.: C299)
Anti-Mouse CD8a (Ly 2.2)-Allophycocyanin (Leinco Prod. No.: C301)
Anti-Mouse CD8a (Ly 2) (All Alleles)-Purified (Leinco Prod. No.: C215)
Anti-Mouse CD8a (Ly 2) (All Alleles)-Allophycocyanin (Leinco Prod. No.: C339)
PN:
Anti-Mouse CD8a (Ly 2.2) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C380)
Anti-Mouse CD8a (Ly 2) (All Alleles) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C375)
Anti-Mouse CD8a (Ly 2.2)-Biotin (Leinco Prod. No.: C298)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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