Product Datasheet
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Anti-Human Adiponectin (ACRP30)
Purified No Carrier Protein
(ADIPOQ, ACDC, ACRP30, APM-1, APM1, GBP28)
Monoclonal Antibody
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Prod. No.:A369
Clone:166126
Isotype:Mouse IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Adiponectin (Clone 166126) recognizes an epitope on Human Adiponectin. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism and insulin sensitivity. Adiponectin expression is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease. Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin and Cadherin-13/T-Cadherin, as well as with several growth factors. Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes. The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human Adiponectin (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Mouse Adiponectin Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A393) at a concentration of approximately 0.5-2.0 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 4 ng/well. A suggested standard for this assay is Recombinant Human Adiponectin (Leinco Prod. No.: A321).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse Adiponectin Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A393)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118)
Recombinant Human Adiponectin (Leinco Prod. No.: A321)
Anti-Human Adiponectin (CT) (Leinco Prod. No.: A268)
Adiponectin (CT) Blocking Peptide (Leinco Prod. No.: A277)
Adiponectin (NT) Blocking Peptide (Leinco Prod. No.: A278)
Anti-Human Adiponectin (NT) (Leinco Prod. No.: A269)
Anti-Mouse Adiponectin (Antigen Aff. Pur.) (Leinco Prod. No.: A355)
Recombinant Mouse Adiponectin (Leinco Prod. No.: A411)
Recombinant Human gAcrp30 (Leinco Prod. No.: A222)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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