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Anti-Human B7 Homolog 2 (B7-H2)
Purified No Carrier Protein
(B7RP-1, B7h, LICOS, GL50)
Monoclonal Antibody
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Prod. No.:B569
Clone:136726
Isotype:Mouse IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human B7-H2 (Clone 136726) recognizes an epitope on Human B7-H2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, or rmB7-H2.

Background:
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human B7-H2 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human B7-H2 is approximately 3.0 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing B7-H2. A suggested method would be to stain cells expressing B7-H2 with 0.25 µl per 1-2.5 x 105 cells in a total staining volume of ≤200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human B7-H2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human B7-H2 is 1-4 µg/ml in the presence of 20 ng/mL of anti-human CD3 and 3 μg/mL of rhB7-H2/Fc, using 72 hour PHA-stimulated human T cells.
Western Blotting: To detect Human B7-H2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human B7-H2 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human B7-H2 (Leinco Prod. No.: B537).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human B7-H2 (Leinco Prod. No.: B537)
Recombinant Human B7-H2/Fc Chimera, CF (Leinco Prod. No.: B528)
Anti-Mouse B7-H2 (Antigen Aff. Pur.) (Leinco Prod. No.: B562)
Anti-Human B7-H2 (Antigen Aff. Pur.) (Leinco Prod. No.: B563)
Anti-Mouse B7-H2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B606)
Anti-Human B7-H2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B607)
Recombinant Mouse B7-H2 (Leinco Prod. No.: B536)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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