Anti-Human β-NGF (Clone 25623)

Purified Recombinant Human β-NGF (Accession # CAA36832)

This monoclonal antibody has been 0.2 µm filtered and lyophilized from DPBS pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. NOTE: Small pack sizes are supplied as a 0.2 µm filtered solution in PBS.

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

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Neutralization: This antibody is useful for neutralization of Human β-NGF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human β-NGF (at 5.0 ng/ml) is 0.1 - 0.4 µg/ml.

Mouse Anti-Human Beta-Nerve Growth Factor (β-NGF) (Clone 25623) recognizes an epitope on Human β-NGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows strong cross-reactivity with rhBDNF, rhNT-3 and rhNT-4. However, this antibody did not neutralize the biological activity of rhBDNF, rhNT-3 or rhNT-4. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human β-NGF. When used in combination with the biotinylated anti-human β-NGF detection antibody (Catalog # BAF256) in sandwich ELISAs, less than 5% cross-reactivity was observed with rrβ-NGF, rmβ-NGF, rhBDNF, rhNT-3 or rhNT-4

Nerve growth factor beta (NGF-beta) is a secreted protein that is important for the differentiation and survival of neurons and dermal keratinocytes.¹ It is also involved in cell proliferation and survival, and it is a mediator of the immune response.² NGF-beta accelerate ulcer healing.³

1. Schenck, K. et al. (2007) Eur J Oral Sci. 115: 344
2. Epplen, JT. et al. (2008) BMC Med Genet. 9: 107
3. Dai, J. et al. (2009) Biomaterials. 30(27):4649-56.