Anti-Human CCL14 (Clone 256409)

Anti-Human CCL14 (Clone 256409)

Product No.: C1604

[product_table name="All Top" skus="C1604"]

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Clone
256409
Target
CCL14
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Chemokine (C-C Motif) Ligand 14, HCC-1, HCC-3
Isotype
IgG2a
Applications
ELISA Cap
,
N
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human CCL14 (Accession # NP_116738)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:H256 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:H257.
Western Blotting: To detect Human CCL14 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CCL14 is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:H257.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human CCL14 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL14 (at 10 ng/ml) is 0.5-2.5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human CCL14 (Clone 256409) recognizes an epitope on Human CCL14. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Details

PubMed
NCBI Gene Bank ID
Elisa Sandwich Protocol
N
General Western Blot Protocol

Formats Available

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Prod No.
Description
C1604
Products are for research use only. Not for use in diagnostic or therapeutic procedures.