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Anti-Human CD11b
DyLight® 680
(Mac-1 Integrin αMβ2)
Monoclonal Antibody
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Prod. No.:C1724
Clone:44
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD11b (Clone 44) recognizes an epitope on Human CD11b. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages.1,2

Background:
Anti-CD11b may be used to study lymphocyte subsets in flow cytometry and in cell adhesion research (Mac-1). The antibody may be used to stain tissue sections which have been acetone fixed and frozen.

Reporter Molecule:
DyLight® 680 conjugated antibodies absorb light maximally around 682 nm (± 4 nm) and fluoresce maximally around 715 nm. DyLight® 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5 and Alexa Fluor 680, and is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CD11b (>98%)
Formulation
This DyLight® 680 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 680 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104; III (1987) Oxford University Press: Code No. 202 and 238; IV (1989) Oxford University Press: Code No. M147
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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