Anti-Mouse/Rat CD126 (IL-6Rα) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Rat CD126 (IL-6Rα) – Purified in vivo GOLD™ Functional Grade
Product No.: I-1185
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Clone D7715A7 (15A7) Target CD126 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-6 Receptor α chain, IL-6R Isotype IgG2b κ Applications FA , FC , in vivo , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen OKT4 hybridoma cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737534 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this D7715A7 (15A7) antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this D7715A7 (15A7) antibody for use in western blotting is 1-10 μg/ml. Additional Reported Applications For Relevant Conjugates ? N For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone D7715A7 (Clone 15A7) recognizes an epitope on mouse CD126. Antigen Distribution CD126 is expressed on activated T and B cells, monocytes, hepatocytes and plasma cells. Background CD126 (IL-6Rα) is an 80 kD type I cytokine receptor α chain that is a member of the Ig superfamily that can form a heterodimer with IL-6Rβ chain to form the high affinity IL-6 receptor. The IL-6/IL-6R complex is a potent pleiotropic cytokine that mediates cell growth and differentiation and has significant involvement in immune response. Inadequate production of IL-6 and IL-6R are thought to play a part in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Antigen DetailsNCBI Gene Bank ID UniProt.org Research Area Cell Biology . Immunology . Innate Immunity . Neuroinflammation . Neuroscience . Signal Transduction . Other Molecules Technical ProtocolsFA   N  |
Formats Available
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Prod No. | Description |
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I-1185 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
