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Anti-Human CD13 (CD13)
Fluorescein (FITC)
(Myeloid Cells)
Monoclonal Antibody
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Prod. No.:C151
Clone:22A5
Isotype:Mouse IgG
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD13 (Clone 22A5) recognizes Human CD13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD13 (Myeloid) recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts.1

Antigen Distribution:
The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.

Background:
Anti-Human CD13 can be used to study myeloid cell functions as well as to immunophenotype leukemias. CD13 is present on basophils from subjects with chronic myeloid leukemia (CML) and on granulocytes from subjects with systemic lupus erythematosus (SLE).

Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 20 µl per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Horton, A. M. et al. (1985) Cancer Res. 45:5663
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Anti-Human CD13 (Myeloid Cells)-Biotin (Leinco Prod. No.: C150)
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Anti-Human CD13 (Myeloid Cells) Purified (Leinco Prod. No.: C149)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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