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Anti-Human CD13
DyLight® 755
(Myeloid Cells)
Monoclonal Antibody
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Prod. No.:C1671
Clone:22A5
Isotype:Mouse IgG
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD13 (Clone 22A5) recognizes an epitope on Human CD13. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.

Background:
Anti-Human CD13 can be used to study myeloid cell functions as well as to immunophenotype leukemias. CD13 is present on basophils from subjects with chronic myeloid leukemia (CML) and on granulocytes from subjects with systemic lupus erythematosus (SLE).

Reporter Molecule:
DyLight® 755 conjugated antibodies absorb light maximally around 754 nm (± 4 nm) and fluoresce maximally around 776 nm. DyLight® 750 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor 750, and has high solubility for maximal incorporation of the dye into the biomolecule of interest. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CD13 (>98%)
Formulation
This DyLight® 755 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 755 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Horton, A. M. et al. (1985) Cancer Res. 45:5663
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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